Research Laboratory at the University of Kansas

Publications

  • Asad, N., Rivera, L., and Timmons, L. (in preparation) Cytoplasmic versus nuclear RNAi mechanisms in transgene-induced gene silencing in Caenorhabditis elegans .
  • Timmons, L., Luna, H., Martinez, J., and Moore, Z., Nagarajan V., Kemege J.M., Asad N. (2014) Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction. FEBS Lett: 588(18)3347-51.
  • Beckert, U., Aw, W. Y., Burhenne, H., Forsterling, L., Timmons, L., and Seifert, R. (2013) The receptor-bound guanylyl cyclase DAF-11 is the mediator of hydrogen peroxide-induced cGMP increase in Caenorhabditis elegans . PLoS ONE 8(8):e72569.
  • Asad, N., Aw, W. Y. and Timmons, L. (2012) Natural and unanticipated modifiers of RNAi activity in Caenorhabditis elegans . PLoS ONE 7(11):e50191.
  • Timmons, L. (2010) Systemic RNAi in Caenorhabditis elegans from the viewpoint of RNA as extracellular signal. pp. 69-92 Extracellular Nucleic Acids, Nucleic Acids and Molecular Biology series, Kikuchi, Y, and Rykova, EY, eds.
  • Han, W., Sundaram, P., Kenjale, H., and Timmons, L. (2008) The Caenorhabditis elegans rsd-2 and rsd-6 genes preserve chromosome integrity during exposure to unfavorable environments Genetics Vol. 178(4): 1875-1893.
  • Sundaram, P., Han, W., Cohen, N., Echalier, B., Albin, J., and Timmons, L. (2008) Caenorhabditis elegans ABCRNAi transporter genes interact genetically with rde-2 and mut-7. Genetics 178(2): 801- 814.
  • Timmons, L. (2007) ABC transporters and RNAi in Caenorhabditis elegans . J. Bioenergetics and Biomembranes 39(5-6): 459-63.
  • Sundaram, P., Echalier, B., Han, W., Hull, D., and Timmons, L. (2006) ATP binding cassette transporters are required for efficient RNAi in Caenorhabditis elegans . Molecular Biology of the Cell 17, 367 8-3688.
  • Timmons, L. (2006) Construction of plasmids for RNA interference and in vitro transcription of double-Stranded RNA. C. elegans: Methods and Applications. (Humana Press) Kevin Strange, ed., pp. 109-118.
  • Timmons, L. (2006) Delivery methods for RNA interference in C. elegans. C. elegans:Methods and Applications. (Humana Press) Kevin Strange, ed., pp. 119-125.
  • Provost, E., Hersperger, G., Timmons, L., Ho W.-Q., Hersperger, E., Alcazar, R., and Shearn, A. (2005) Loss of function mutations in a glutathione-S-transferase suppress the prune-Killer of prune lethal interaction. Genetics 172(1): 207-19.
  • Timmons, L. (2004) Endogenous inhibitors of RNA interference in C. elegans. BioEssays26(7) 715-8.
  • Hull, D. and Timmons, L. (2004) Methods for delivery of double-stranded RNA into C. elegans” in Methods Mol Biol: RNA interference, editing, and modification. (Humana Press) Jonatha Gott, ed. 265:23-58.
  • Timmons, L., Tabara, H., Mello, C., Fire, A. (2003) Inducible systemic RNA silencing in Caenorhabditis elegans . Molecular Biology of the Cell 14: 2972-83.
  • Timmons, L. (2002) The long and short of siRNAs. Molecular Cell 10: 435-437..
  • Sijen T, Fleenor J, Simmer F, Thijssen KL, Parrish S, Timmons L., Plasterk RH, Fire A. (2001) On the role of RNA amplification in dsRNA-triggered gene silencing. Cell Nov 16;107(4):465-76.
  • Timmons, L. and Fire, A. (2001) Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103-12.
  • Timmons, L. and Shearn, A. (2000) Role of AWD/nucleoside diphosphate kinase in Drosophila development. Journal of Bioenergenetics and Biomembranes 32:293-300.
  • Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A., and Mello, C. C. (1999) The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99:123-132.
  • Timmons, L. and Fire, A. (1998) Specific genetic interference from ingested double-stranded RNA. Nature 395(6705):854.
  • Timmons, L., Becker, J., Barthmaier, P., Fyrberg, C., Shearn, A., and Fyrberg, E. (1997) Green fluorescent protein/Beta-galactosidase double reporters for visualizing Drosophilagene expression patterns. Developmental Genetics 20:338-347.
  • Timmons, L. and Shearn, A. (1997) Prune/Killer of prune: A conditional dominant lethal interaction in Drosophila. Advances in Genetics 35:207-252.
  • Timmons, L. and Shearn, A. (1996) Germline transformation using a prune cDNA rescues prune/Killer of prune lethality and the prune eye color phenotype. Genetics 144:1589-1600.
  • Xu, J., Liu, L-Z., Deng, X., Timmons, L., Hersperger, E., Steeg, P. S., Veron, M., and Shearn, A. (1996) The enzymatic activity of Drosophila AWD/NDP kinase is necessary but not sufficient for its biological function. Developmental Biology 177:544-557.
  • Timmons, L., Xu, J., Hersperger, G., Deng, X. F., and Shearn, A. (1995) Point mutations in awdKpn that revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis. J. Biol. Chem. 270:23021-23030.
  • Timmons, L., Hersperger, E., Woodhouse, E., Xu, J., Liu, L.-Z., and Shearn, A. (1993) The expression of the Drosophila awd gene during normal development and in neoplastic brain tumors caused by lgl mutations. Developmental Biology 158:364-379.
  • Scandella, D., Timmons, L., Mattingly, M., Trabold, N., and Hoyer, L. W. (1992) A soluble recombinant factor VIII fragment containing the A2 domain binds to some human anti-factor VIII antibodies that are not detected by immunoblotting. Thrombosis and Haemostasis67:665-671.
  • Scandella, D., Mahoney, S. D., Mattingly, M., Roeder, D., Timmons, L., and Fulcher, C. A. (1988) Epitope mapping of human factor VIII inhibitor antibodies by deletion analysis of factor VIII fragments expressed in Eschericia coli. Proc. Natl. Acad. Sci. USA 85:6152-6156.